Loading Control Antibodies
Loading control proteins are essential for the accurate interpretation of western blot data. Loading control antibodies allow the verification of equal protein loading between samples. Ideal loading controls are expressed constitutively and at high levels with low variability between cell lines and experimental conditions. The loading control should be at a different molecular weight than the protein of interest this allows the bands to be visually distinguishable.
Featured Loading Control Antibodies from Bioss:
|Beta Tubulin||bs-4511R||WB, IHC-P, IF(IHC-P)||Human, Mouse, Rat||Rabbit Polyclonal||11|
|Beta Actin||bs-0061R||WB, IF(ICC)||Human, Mouse, Rat, Bovine, Pig, Rabbit||Rabbit Polyclonal||465|
|BrdU||bsm-0917M||IHC-P, IF(IHC-P)||BrdU||Mouse Monoclonal||5|
|COX4||bs-10257R||WB, FCM, IF(ICC)||Human, Mouse, Rat||Rabbit Polyclonal||6|
|GAPDH||bs-2188R||WB, IHC-P, IF(IHC-P)||Human, Mouse, Rat||Rabbit Polyclonal||69|
|Histone H3||bs-0349R||WB, FCM, IHC-P, IF(IHC-P)||Human, Mouse, Rat, Pig||Rabbit Polyclonal||21|
|Lamin A/C||bs-1839R||WB, IHC-P, IF(IHC-P), IF(ICC)||Human, Mouse, Rat||Rabbit Polyclonal||4|
|PCNA||bs-0754R||WB, IHC-P, IF(IHC-P)||Human, Mouse, Rat||Rabbit Polyclonal||14|
|VDAC 1||bs-1461R||WB, IHC-P, IF(IHC-P)||Human, Mouse, Rat||Rabbit Polyclonal||6|
|Vinculin||bs-6640R||WB, IHC-P, IF(IHC-P), IF(ICC)||Human, Mouse, Rat||Rabbit Polyclonal||7|
Loading Control Spotlights
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.