Loading Control Antibodies

Loading control proteins are essential for the accurate interpretation of western blot data. Loading control antibodies allow the verification of equal protein loading between samples. Ideal loading controls are expressed constitutively and at high levels with low variability between cell lines and experimental conditions. The loading control should be at a different molecular weight than the protein of interest this allows the bands to be visually distinguishable.


Featured Loading Control Antibodies from Bioss:

Antibody Catalog number Applications Reactivity Type Publications
Beta Tubulin bs-4511R WB, IHC-P, IF(IHC-P) Human, Mouse, Rat Rabbit Polyclonal 11
6 HIS-TAG bsm-0287M WB, IHC-P, IF(IHC-P), IF(ICC) HHHHHH tag Mouse Monoclonal 17
Beta Actin bs-0061R WB, IF(ICC) Human, Mouse, Rat, Bovine, Pig, Rabbit Rabbit Polyclonal 465
BrdU bsm-0917M IHC-P, IF(IHC-P) BrdU Mouse Monoclonal 5
COX4 bs-10257R WB, FCM, IF(ICC) Human, Mouse, Rat Rabbit Polyclonal 6
GAPDH bs-2188R WB, IHC-P, IF(IHC-P) Human, Mouse, Rat Rabbit Polyclonal 69
GFP TAG bs-0639R IHC-P, IF(IHC-P), IF(ICC) GFP Rabbit Polyclonal 3
GST TAG bs-2735R WB, IHC-P, IF(IHC-P) GST tag Rabbit Polyclonal 5
Histone H3 bs-0349R WB, FCM, IHC-P, IF(IHC-P) Human, Mouse, Rat, Pig Rabbit Polyclonal 21
Lamin A/C bs-1839R WB, IHC-P, IF(IHC-P), IF(ICC) Human, Mouse, Rat Rabbit Polyclonal 4
MBP TAG bs-0380R WB, IHC-P, IF(IHC-P) Human, Mouse, Rat, MBP-Tag Rabbit Polyclonal 6
MYC TAG bs-4963R IHC-P, IF(IHC-P), IF(ICC) Human, Mouse, Rat Rabbit Polyclonal 7
PCNA bs-0754R WB, IHC-P, IF(IHC-P) Human, Mouse, Rat Rabbit Polyclonal 14
VDAC 1 bs-1461R WB, IHC-P, IF(IHC-P) Human, Mouse, Rat Rabbit Polyclonal 6
Vinculin bs-6640R WB, IHC-P, IF(IHC-P), IF(ICC) Human, Mouse, Rat Rabbit Polyclonal 7

Loading Control Spotlights

 

bsm-50179M WB

Alpha Tubulin - bsm-50179M

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

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bsm-33037M IF(IHC-P)

COX4 - bsm-33037M

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

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bsm-52251R IHC-P

VDAC1 - bsm-52251R

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

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