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Knockout Validated Antibodies

As a dedicated researcher, you deserve extraordinary products in support of your studies. Bioss understands your concern and strives to meet your expectations every day, which is why we initiated knockout (KO) validation program on our selected products.

With the advance of biological discoveries, not only researchers demand more from their antibodies, but also the companies that produce them. Bioss seeks to improve the user experience by providing top quality product testing. In this pursuit, we recognize the need for more tailored validations of our antibodies against knockout, knock-in, and mutated targets. Knockout validated antibodies allow for increased experimental reliability and reproducibility. During the validation process, antibodies tested against wildtype cell lines are compared to those tested against knockout cell lines that don't express the target protein. A specific antibody should detect only the target from the wildtype and find nothing from the knockout. This process provides a reliable negative control for the antibody's target specificity.

Through a new partnership with EdiGene Inc. (http://www.edigene.com), a leader in genome-edited human cell lines, we have selected many most popular knockout lysates for our antibody validation program, with more expected in the future. Data gathered through such testing will afford a more robust understanding of each antibody's properties and allow researchers to make more informed purchases.

All listed applications are Guaranteed.

Featured KO-Validated Antibodies from Bioss:

Target Cat # Applications Target Cat # Applications
ACE2 Rabbit Polyclonal Antibody bs-23443R WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC P21 Polyclonal Antibody bs-55176R WB, ELISA, IHC-P, IHC-F
ACE2 rabbit polyclonal antibody bs-23444R WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC PARP1 Polyclonal Antibody bs-55164R WB, ELISA
Bad (Ser118) Polyclonal Antibody bs-5216R WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC PDCD4 Polyclonal Antibody bs-55166R WB, ELISA
Beclin 1 Polyclonal Antibody bs-55024R WB PRKAR2A Polyclonal Antibody bs-55176R WB, ELISA
CD44 Polyclonal Antibody bs-55039R WB, IF(ICC), IF PTPN1 Polyclonal Antibody bs-55182R WB, ELISA
CK18 Polyclonal Antibody bsm-33050M WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC) RHOC Polyclonal Antibody bs-55187R WB, ELISA, IHC-P, IHC-F, IF
DNMT1 Polyclonal Antibody bs-55056R WB, IF(ICC), IP, IF SCD Polyclonal Antibody bs-55193R WB, ELISA, IHC-P, IHC-F, IF
EGFR Monoclonal Antibody bsm-33293M WB SIRT3 Polyclonal Antibody bs-55199R WB, ELISA, IHC-P, IHC-F
ERK2 Polyclonal Antibody bs-55093R WB, ELISA SMARCA4 Polyclonal Antibody bs-55203R WB, ELISA
GSK3B (3A6) Monoclonal Antibody bsm-33136M WB, IHC-P, IF(IHC-P) SQSTM1/p62 Polyclonal Antibody bs-55207R WB, ELISA
HDAC1 Polyclonal Antibody bs-0608R WB, ELISA, IHC-P, IHC-F, IP, IF STAT3 (3F5) Monoclonal Antibody bsm-33218M WB, IHC-P
HMGB1 Polyclonal Antibody bs-55098R WB, IHC-P, IF STAT3 (5H4)monoclonal antibody bsm-33223M WB, IHC-P
HSP90 beta Polyclonal Antibody bs-55138R WB STAT3 Polyclonal Antibody bs-55208R WB, IHC-P, IHC, IF
ICAM1 Polyclonal Antibody bs-55141R WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC STK4 Polyclonal Antibody bs-55209R WB, ELISA
Lamin B1 Polyclonal Antibody bsm-33117M WB, ELISA, IHC-P, IHC-F TAGLN2 Polyclonal Antibody bs-55211R WB, ELISA, IF
N-Cadherin Polyclonal Antibody bs-2590R WB, ELISA TMP21 Polyclonal Antibody bs-11262R WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
NDRG1 Polyclonal Antibody bs-55160R WB, ELISA Vimentin (3B7) Monoclonal Antibody bsm-33170M WB
NFKB p65 (7G6) Monoclonal Antibody bs-55164R WB Vimentin Polyclonal Antibody bs-0756R WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
NRSF/REST Polyclonal Antibody bs-55166R WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC) Vimentin Polyclonal Antibody bs-8533R WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC


Featured Spotlight Items

bs-0756R WB-KO

Vimentin - bs-0756R

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.




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bs-0608R WB-KO


ICAM1 - bs-0608R

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).




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bs-1339R WB-KO

CK18 - 1339R

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.




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